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\Cellular Rejuvenation: Extended replicative lifespan in cultured cells from 34 to 44 passages (Lab Study)

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For CYP3A4 inhibition testing, ketoconazole (MilliporeSigma, catalog: PHR1385) was prepared at 2 final concentration in culture medium and added in equal volume to each well of a 384-well plate for 24 h

In the blend they are already pre-mixed in one vial, so co-injection is the intended use and no separate mixing is needed

Materials and Methods Animals Study protocols were conducted in male albino Wistar rats, body weight 200 g, 13 weeks old, in-house bredanimal facility, Department of Pharmacology, School of Medicine, Zagreb, Croatia

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