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doi: 10.1074/jbc.272.43.27218 53 MaedaNFunahashiTHibuseTNagasawaAKishidaKKuriyamaHet al

Cells were cultured in Dulbeccos modified Eagles medium (DMEM) (Gibco, NY, USA) supplemented with 10 % foetal bovine serum (FBS) (Gibco, NY, USA) and were maintained in a humidified incubator at 37 C and 5 % CO 2

Briefly, 4 mg of MIA were dissolved in 40 L of sterile saline and this volume of solution was injected intraarticularly through the patellar ligament of the left knee joint of each rat that had been anesthetized using 50 mg/kg sodium pentobarbital by intraperitoneal injection

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These personal experiences are pushing researchers to look beyond weight management and explore whether these drugs might help with a wider range of health concerns

L-Arginine is an amino acid that is a precursor to nitric oxide