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Large-scale epidemiological surveys in Chinese commercial farms indicated that under conditions of high temperaturehumidity or organic bedding, milk frequently tested positive for pathogens such as Escherichia coli and Klebsiella spp., which ascended via the teat canal, thereby shifting the etiological profile toward environmental pathogens 132

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In recent years, several protocols have been developed using Agrobacterium tumefaciens and Agrobacterium rhizogenes as gene delivery systems ( Figure 6 )