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Quantification of lipogenesis gene expression To quantify gene expression, we used the Pure Link RNA Mini kit (12183025, Invitrogen, USA), Hilden, Germany) to extract total RNA from untreated and treated HepG2 cells and used High-Capacity cDNA Reverse Transcription kit (4368813, Applied Biosystems, Foster City, CA, USA) and 2 g total RNA to prepare cDNA

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Several injectable medications have received FDA approval for chronic weight management, though none carry specific labeling for use in post-bariatric surgery patients

Analysis of microRNA expression after glutamine intervention in acute renal ischemia-reperfusion injury

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These factors collectively contribute to myocardial cell injury and death, affecting both systolic and diastolic cardiac function while promoting adverse myocardial remodeling and eventual heart failure progression (19)